Comparison of RheoScan with LORCA and Rheodyn SSD
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Comparison of three commercially available ektacytometers with different shearing geometries

In December 2008, the International Society for Clinical Hemorheology organized a workshop to evaluate and compare
three ektacytometer instruments for measuring deformability of red blood cells (RBC): LORCA (Laser-assisted Optical
Rotational Cell Analyzer, RR Mechatronics, Hoorn, The Netherlands), Rheodyn SSD (Myrenne GmbH, Roetgen, Germany)
and RheoScan-D (RheoMeditech, Seoul, Korea). Intra-assay reproducibility and biological variation were determined using
normal RBC, and cells with reduced deformability (i.e., 0.001–0.02% glutaradehyde (GA), 48◦C heat treatment) were employed
as either the only RBC present or as a sub-population. Standardized difference values were used as measure of the
power to detect differences between normal and treated cells. Salient results include: (1) All instruments had intra-assay variations
below 5% for shear stress (SS) > 1 Pa but a sharp increase was found for Rheodyn SSD and RheoScan-D at lower
SS; (2) Biological variation was similar and markedly increased for SS < 3&#8211;5 Pa; (3) All instruments detected GA-treated
RBC with maximal power at 1&#8211;3 Pa, the presence of 10% or 40% GA-modified cells, and the effects of heat treatment. It is
concluded that the LORCA, Rheodyn SSD and RheoScan-D all have acceptable precision and power for detecting reduced
RBC deformability due to GA treatment or heat treatment, and that the SS range selected for the measurement of deformability
is an important determinant of an instrument’s power.
File : 2009-5-Biorheol-Three Eckta.pdf